In vitro production of foregut stem cells
US10000740B2 · kind B2 · utility
12Cited by
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12Claims
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Key dates
| Filing date | Oct 6, 2014 |
| Grant date | Jun 19, 2018 |
| Priority date | — |
| Expiry date | Oct 6, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/45
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to the differentiation of pluripotent cells (PSCs) into foregut stem cells (FSCs) using a definitive endoderm induction medium comprising a TGFfi ligand, fibroblast growth factor (FGF), bone morphogenetic protein (BMP) and a PI3K inhibitor to differentiate the pluripotent cells into definitive endoderm cells and a foregut induction medium comprising a TGFβ ligand to differentiate the definitive endoderm cells into foregut stem cells (FSCs). Methods of differentiation, populations of foregut stem cells, culture media and kits are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.