Engineered enzyme having acetoacetyl-CoA hydrolase activity, microorganisms comprising same, and methods of using same
US10000744B2 · kind B2 · utility
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Key dates
| Filing date | Sep 23, 2014 |
| Grant date | Jun 19, 2018 |
| Priority date | — |
| Expiry date | Nov 12, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/02011
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosure provides engineered enzymes that are capable of mediating the conversion of acetoacetyl-CoA to acetoacetate that do not react with the same order of magnitude with acetyl-CoA as they do with acetoacetyl-CoA (e.g., the engineered enzymes have a specific acetoacetyl-CoA hydrolase activity at least 10× higher than its acetyl-CoA hydrolase activity). Additionally, the disclosure provides modified microorganisms that comprise the engineered enzymes disclosed herein and methods of using same.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.