Yeast alleles involved in maximal alcohol accumulation capacity and tolerance to high alcohol levels

US10000759B2 · kind B2 · utility

Assignees

• VIB VZW · BE
• Katholieke Universiteit Leuven, KU LEUVEN R&D · BE

Inventors

• Johan Thevelein · Blanden, BE
• Annelies Goovaerts · Putte, BE
• Françoise Dumortier · Blanden, BE
• Maria Remedios Foulquie-Moreno · Brussels, BE
• Steve Swinnen · Zichem, BE
• Thiago Martins Pais · Sinop, BR

Key dates

• Filing date: Apr 15, 2014
• Grant date: Jun 19, 2018
• Expiry date: Apr 15, 2034

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure relates to a specific yeast allele of KIN3 that is involved in maximal alcohol accumulation and/or in tolerance to high alcohol levels. Preferably, the alcohol is ethanol. In a preferred embodiment, this specific allele is combined with specific alleles of ADE1 and/or VPS70. More specifically, the disclosure relates to the use of these alleles for the construction and/or selection of high alcohol tolerant yeasts, by stacking of positive alleles, or the selection and construction of low alcohol producing yeasts by stacking of negative alleles.