Materials and methods for rapid visualization of NAD(P)H
US10000790B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 28, 2015 |
| Grant date | Jun 19, 2018 |
| Priority date | — |
| Expiry date | Apr 26, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The subject invention provides materials and methods for detecting the presence of an electron donor. In a specific embodiment, the device detects the presence of dihydronicotinamide adenine dinucleotide (NADH) via a colorimetric change output by the sensing device. In another specific embodiment, the presence of an enzyme capable of catalyzing, or an agent capable of inhibiting, the production of NADH can also be detected by a colorimetric readout using the same device. In some embodiments, dihydronicotinamide adenine dinucleotide phosphate (NADPH) can also be detected using the device provided herein. Advantageously, preferred embodiments of the subject invention provide a low-cost, sensitive device for monitoring the presence of critical biological analytes in a variety of applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.