Droplet storage method
US10000794B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jun 13, 2014 |
| Grant date | Jun 19, 2018 |
| Priority date | — |
| Expiry date | Aug 10, 2035 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0893
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method of storing a stream of droplets at least some of which comprise one or more single nucleotides and/or oligonucleotides, and a droplet fluid is provided. It is characterized by the step of introducing each droplet sequentially onto a surface of a substrate at a corresponding unique location and further characterized in that the stream of droplets is prepared by a process which includes the steps of generating an ordered stream of nucleotides from the analyte by progressive pyrophosphorolysis or exo nucleolysis and capturing each nucleotide in a corresponding droplet. The method can advantageously be used in association with microdroplet droplet sequencers and an analysis unit in which the sequence of nucleotides in a precursor polynucleotide analyte is determined using fluorescence spectroscopy. A device for carrying out the method is also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.