Spatially addressable molecular barcoding
US10002316B2 · kind B2 · utility
220Cited by
115References
26Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 2, 2017 |
| Grant date | Jun 19, 2018 |
| Priority date | — |
| Expiry date | Aug 2, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.