Method of segmenting single neuron images with high-dynamic-range thresholds and computer readable storage medium thereof
US10002425B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Dec 6, 2016 |
| Grant date | Jun 19, 2018 |
| Priority date | — |
| Expiry date | Dec 6, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2210/41
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
The method of segmenting single neuron images with high-dynamic-range thresholds of the present invention includes (a) preparing a biological tissue sample containing neurons and performing imaging to this sample to obtain a three-dimensional raw neuroimage; (b) deleting voxels in the three-dimensional raw neuroimage with signal intensities below a first signal intensity threshold to obtain a first thresholded image; (c) tracing the first thresholded image to obtain a first traced image; (d) calculating a structural importance score of every voxel in the first traced image to obtain a first structural importance score of every voxel; (e) gradually increasing the signal intensity threshold and repeating (b), (c) and (d) n−1 times; (f) summing up all the n structural importance scores of every voxel; (g) deleting voxels with summed structural importance score smaller than a pre-determined value from the raw image to obtain the segmented single neuron.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.