Methods and compositions for producing hepatocyte-like cells

US10004767B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Gary Peltz · Redwood City, US
• Dan Xu · Nanjing, CN

Key dates

• Filing date: Sep 17, 2014
• Grant date: Jun 26, 2018
• Expiry date: Sep 17, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/90
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods are provided for producing a population of hepatocyte-like cells (iHeps) from a population of adipocyte-derived stem cells (ASCs). Aspects of the methods include placing a population of ASCs into a three dimensional culture (e.g., hanging drop suspension culture, high density culture, spinner flask culture, microcarrier culture, etc.), and contacting the cells with a first and second culture medium. Also provided are methods of treating an individual, which include producing a population of iHeps from a population of ASCs, and administering an effective number of iHeps into the individual. Kits for practicing the methods are also described herein.