Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation

US10005080B2 · kind B2 · utility

Assignees

• GenMark Diagnostics, Inc. · US
• Advanced Liquid Logic, Inc. · US

Inventors

• Jon Faiz Kayyem · Pasadena, US
• Sean Ford · Oceanside, US
• Vamsee K. Pamula · Durham, US
• Vijay Srinivasan · Durham, US
• Scott Corey · Hydes, US
• Brian Murphy · Baltimore, US
• David W. Wright · Littleton, US
• Dominic Aiello · Denver, US

Key dates

• Filing date: Nov 11, 2014
• Grant date: Jun 26, 2018
• Expiry date: Oct 4, 2035

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2400/0683
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.