Patent · US Active

Lyase and lyase-encoding DNA, vectors containing the DNA, and method for the asymmetric synthesis of (S)-phenylacetylcarbinol

US10006061B2 · kind B2 · utility

1Cited by

3References

7Claims

0Family size

Assignee

Forschungszentrum Jülich GmbH · DE

Inventors

Doerte Rother · Gerstungen, DE
Martina Pohl · Aachen, DE
Torsten Sehl · Offenburg, DE
Lisa Marx · Seeheim-Jugenheim, DE
Robert Westphal · Opfingen, DE

Key dates

Filing date	Aug 14, 2015
Grant date	Jun 26, 2018
Priority date	—
Expiry date	Aug 14, 2035

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y401/01001
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a lyase and a lyase-encoding DNA, to vectors containing the DNA and to a method for the asymmetric synthesis of (S)-phenylacetylcarbinol. According to the invention, a lyase is provided, in which tryptophan is replaced with an amino acid at position 543 in protein ApPDC-E469G, said protein being modified with respect to the wild type of Aceobacter pasteurianus, or in which it is less space-filling than tryptophan. According to the invention, deoxyribonucleic acids are furthermore provided, which encode the lyase. (S)-phenylacetylcarbinol can be produced with the lyase according to the invention from the educts benzaldehyde and pyruvate or acetaldehyde with an enantiomeric excess of at least 94%.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.