Nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal for increasing the expression of an encoded tumour antigen

US10010592B2 · kind B2 · utility

Assignee

• CureVac AG · DE

Inventors

• Andreas Thess · Aspenhausiedlung, DE
• Thomas SCHLAKE · Gundelfingen, DE
• Jochen Probst · Wolfschlugen, DE

Key dates

• Filing date: Feb 15, 2013
• Grant date: Jul 3, 2018
• Expiry date: May 23, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/50
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to a nucleic acid sequence, comprising or coding for a coding region, encoding at least one peptide or protein comprising a tumor antigen or a fragment, variant or derivative thereof, at least one histone stem-loop and a poly(A) sequence or a polyadenylation signal. Furthermore the present invention provides the use of the nucleic acid for increasing the expression of said encoded peptide or protein. It also discloses its use for the preparation of a pharmaceutical composition, especially a vaccine, e.g. for use in the treatment of cancer or tumor diseases. The present invention further describes a method for increasing the expression of a peptide or protein comprising a tumor antigen or a fragment, variant or derivative thereof, using the nucleic acid comprising or coding for a histone stem-loop and a poly(A) sequence or a polyadenylation signal.