Patent · US Active

Cleavable hairpin primers

US10011861B2 · kind B2 · utility

1Cited by
2References
10Claims
0Family size

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Inventors

Key dates

Filing dateAug 14, 2015
Grant dateJul 3, 2018
Priority date—
Expiry dateMay 9, 2036

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6827
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.