Method for relative quantification of changes in DNA methylation, using combined nuclease, ligation, and polymerase reactions
US10011862B2 · kind B2 · utility
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25Claims
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Key dates
| Filing date | Mar 13, 2014 |
| Grant date | Jul 3, 2018 |
| Priority date | — |
| Expiry date | Jul 31, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/154
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to methods for identifying the presence of one or more methylated or unmethylated target nucleotide sequences in a sample that involve a nuclease-ligation reaction. In some embodiments, the ligation products formed in the nuclease-ligation process of the present invention are subsequently amplified using a polymerase chain reaction. The ligated product sequences or extension products thereof are detected, and the presence of one or more methylated or unmethylated target nucleotide sequences in the sample is identified based on the detection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.