Fluorescence sensor for target analysis, kit for target analysis, and target analysis method using same
US10012631B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 27, 2014 |
| Grant date | Jul 3, 2018 |
| Priority date | — |
| Expiry date | Jun 27, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/3519
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is intended to provide a novel fluorescence sensor for target analysis, a kit for target analysis, and a target analysis method using the same.The fluorescence sensor for target analysis according to the present invention includes a nucleic acid molecule that includes a G-quartet-forming nucleic acid region (D) that forms a G-quartet and a binding nucleic acid region (A) that binds to a target. In the absence of a target, formation of a G-quartet in the G-quartet-forming nucleic acid region (D) is inhibited. In the presence of a target, the target comes into contact with the binding nucleic acid region (A), the G-quartet is formed in the G-quartet-forming nucleic acid region (D) due to the contact, the G-quartet-forming region (D) and porphyrin forms a complex, and the complex generates fluorescence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.