Methods of expanding and assessing B cells and using expanded B cells to treat disease

US10017739B2 · kind B2 · utility

Assignee

• Duke University · US

Inventors

• Thomas F. Tedder · Durham, US
• Ayumi Yoshizaki · Durham, US
• Tomomitsu Miyagaki · Durham, US
• Evgueni Kountikov · Durham, US
• Jonathan C. Poe · Durham, US

Key dates

• Filing date: Mar 12, 2013
• Grant date: Jul 10, 2018
• Expiry date: Mar 12, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2501/599
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided herein are methods of expanding B cells, and in particularly B10 cells capable of producing IL-10, ex vivo. The methods include incubation of harvested B cells in the presence of IL-21. Compositions comprising the ex vivo expanded B cells and methods of using the expanded B cell-containing compositions to treat diseases or conditions are also provided. Methods of assessing B10 cell function in a subject are also provided.