Modified polymerases for improved incorporation of nucleotide analogues
US10017750B2 · kind B2 · utility
20Cited by
1References
21Claims
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Key dates
| Filing date | Aug 25, 2016 |
| Grant date | Jul 10, 2018 |
| Priority date | — |
| Expiry date | Aug 25, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogs bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.