Multicomponent quantitative analysis method using chromatography
US10018602B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 27, 2014 |
| Grant date | Jul 10, 2018 |
| Priority date | — |
| Expiry date | Jul 20, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/7206
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A number of analytes are divided into groups based on at least either the elution position determined by silica gel column chromatography under the same condition or the partition ratio in a hexane-acetonitrile partition method. To each group, a compound obtained by labeling one of the compounds in the group by deuterium and/or carbon is assigned as the surrogate. After those surrogates are added as internal standard substances to a standard sample for the creation of calibration curves, a GC/MS analysis for the sample is performed and a calibration curve is created for each analyte. In the measurement of an unknown sample, the same set of surrogates are added to the sample and the GC/MS analysis is performed. A quantitative determination processor determines the quantity of each analyte by comparing a peak area ratio calculated from the analysis result with a calibration curve read from the database.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.