System and method for blood vessel analysis and quantification in highly multiplexed fluorescence imaging
US10019796B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 20, 2016 |
| Grant date | Jul 10, 2018 |
| Priority date | — |
| Expiry date | Sep 20, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2211/404
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
Disclosed are novel computer-implemented methods for creating a blood vessel map of a biological tissue. The methods comprise the steps of, accessing image data corresponding to multi-channel multiplexed image of a fluorescently stained biological tissue manifesting expression levels of a primary marker and at least one auxiliary marker of blood vasculature, and extracting features of blood vessels using the primary marker as an input to create a single channel segmentation of the blood vessels. The method further comprises the steps of extracting features of blood vessels using the auxiliary marker to create auxiliary channels as a second input and apply multi-channel blood vessel enhancement. Blood vessel maps are created using the features and tracing the blood vasculature by iteratively extending the centerlines of the initial segmentation using statistical models and geometric rules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.