Method for providing cytoplasmic male sterile Petroselinum crispum plants, cytoplasmic male sterile Petroselinum crispum plants and seeds and plant parts thereof
US10021848B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 22, 2012 |
| Grant date | Jul 17, 2018 |
| Priority date | — |
| Expiry date | Feb 6, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/02
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
The present invention relates to methods for providing cytoplasmic male sterile Petroselinum plants, cytoplasmic male sterile Petroselinum crispum plants and to seeds, cells, tissues and plant parts thereof. Specifically the present invention relates to a method for providing a cytoplasmic male sterile Petroselinum crispum plant comprising: (a) providing first protoplasts obtained from a plant selected from the group consisting of Daucus carota L., Foeniculum vulgare Mill., Apium graveolens L., and Pastinaca sativa L., wherein the first protoplasts have a substantially inactivated nuclear genome and a substantially non-modified cytoplasm; (b) providing second protoplasts obtained from Petroselinum crispum wherein the second protoplasts have a substantially inactivated cytoplasm and a substantially non-modified nuclear genome; (c) fusing said first and second protoplasts; and (d) obtaining from the fusion product of said first and second protoplasts a cytoplasmic male sterile Petroselinum crispum plant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.