Labeled nucleotide analogs having protein shields

US10023605B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Keith Bjornson · Fremont, US
• Jeremiah Hanes · Woodside, US
• Erik Miller · Seattle, US
• Satwik Kamtekar · Mountain View, US
• Lubomir Sebo · Redwood City, US
• Louis Brogley · Santa Cruz, US

Key dates

• Filing date: Jun 1, 2015
• Grant date: Jul 17, 2018
• Expiry date: Aug 13, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2563/131
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Compositions, methods, and systems are provided for nucleotide analogs comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Nucleotide analogs of the invention have a protein shield between the dye moieties and nucleotide moieties of the analog. The nucleotide analogs have two avidin proteins connected by a central dye component, and each avidin protein is further connected to a nucleotide component. The protein can prevent the direct interaction of the dye moiety with the enzyme carrying out nucleotide synthesis preventing photodamage to the enzyme. The nucleotide analogs of the invention can have multiple dyes and multiple nucleotide moieties.