Purification method of virus-like particles
US10023844B2 · kind B2 · utility
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4References
7Claims
0Family size
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Key dates
| Filing date | May 21, 2014 |
| Grant date | Jul 17, 2018 |
| Priority date | — |
| Expiry date | Oct 10, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/16051
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention aims at establishing a novel means for purifying norovirus VLPs. There is provided a purification method of norovirus virus-like particles, comprising: contacting solution containing norovirus virus-like particles with support for hydroxyapatite chromatography, binding the virus-like particles to the support, subsequently washing the support with buffer, and then eluting the virus-like particles from the support with buffer containing phosphate, wherein the phosphate concentration of the buffer used for elution is less than 10 mM.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.