Methods of making modified viral genomes

US10023845B2 · kind B2 · utility

Assignee

• The Research Foundation for The State University of New York · US

Inventors

• Eckard Wimmer · Port Jefferson, US
• Steve Skiena · Setauket, US
• Steffen Mueller · Pforzheim, DE
• Bruce Futcher · Stony Brook, US
• Dimitris Papamichail · Pennington, US
• John Robert Coleman · New York, US
• Jeronimo Cello · Port Jefferson, US

Key dates

• Filing date: Sep 7, 2016
• Grant date: Jul 17, 2018
• Expiry date: Sep 7, 2036

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.