Patent · US Active

Compositions and methods for making selenocysteine containing polypeptides

US10023893B2 · kind B2 · utility

4Cited by

5References

20Claims

0Family size

Assignee

Yale University · US

Inventors

Dieter Soll · Guilford, US
Caroline Aldag · Mannheim, DE
Michael Hohn · Scotch Plains, US

Key dates

Filing date	Sep 23, 2016
Grant date	Jul 17, 2018
Priority date	—
Expiry date	Sep 27, 2036

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2310/531
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Non-naturally occurring tRNASec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNASec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNASec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNASec.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.