Methods for dye selection for protein melt temperature determinations
US10024863B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 14, 2014 |
| Grant date | Jul 17, 2018 |
| Priority date | — |
| Expiry date | Mar 14, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6803
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.