Mammalian cell culture processes for protein production
US10030064B2 · kind B2 · utility
3Cited by
18References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 1, 2016 |
| Grant date | Jul 24, 2018 |
| Priority date | — |
| Expiry date | Jan 27, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2511/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise the addition of glucocorticoid compound during the culturing period. The addition of glucocorticoid compound sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.