Methods for preparing inactivated rotavirus

US10030232B2 · kind B2 · utility

Assignee

• Elanco Tiergesundheit AG · CH

Inventors

• Brent E. Dekker · Larchwood, US
• John Herbert · Fishers, US
• Michael J. Reimnitz · Larchwood, US

Key dates

• Filing date: Jul 18, 2014
• Grant date: Jul 24, 2018
• Expiry date: Jul 18, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2720/12361
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Disclosed are methods for production of inactivated rotavirus directly from cell culture supernatants containing active or infectious rotavirus (e.g., bovine rotavirus), without prior isolation or purification of the rotavirus from the cell culture supernatants. In some embodiments, inactivated rotavirus (e.g., bovine rotavirus) is produced by heating a volume of culture supernatant to at least a temperature at which the rotavirus is inactivated. In some embodiments, the rotavirus (e.g., bovine rotavirus) is inactivated once the entire volume is heated to at least a temperature at which the rotavirus is inactivated. In some embodiments, the temperature of the cell culture supernatant is maintained for a suitable amount of time, which may be determined using any of the tests described herein. Inactivated rotavirus (e.g., bovine rotavirus) may then be isolated from the culture supernatant.