Methods and kits for capturing sperm nucleic acids

US10030241B2 · kind B2 · utility

Assignee

• General Electric Company · US

Inventors

• Patrick McCoy Spooner · Slingerlands, US
• Peter James Tatnell · Cardiff, GB
• Jeffrey Kenneth Horton · Cardiff, GB
• John Richard Nelson · Fryeburg, US
• Michael John Gerdes · Albany, US
• Suzana Kiel · Göttingen, DE
• Ralf Lenigk · Niskayuna, US
• Alexander Schenk · Waiblingen, DE
• Wei Sun · Brookline, US

Key dates

• Filing date: May 10, 2016
• Grant date: Jul 24, 2018
• Expiry date: May 10, 2036

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/124
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.