Methods for producing a complex transgenic trait locus
US10030245B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 22, 2012 |
| Grant date | Jul 24, 2018 |
| Priority date | — |
| Expiry date | Apr 22, 2034 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A40/146
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.