Method and product for localized or spatial detection of nucleic acid in a tissue sample

US10030261B2 · kind B2 · utility

Assignee

• Spatial Transcriptomics AB · SE

Inventors

• Jonas Frisen · Stockholm, SE
• Patrik Stahl · Stockholm, SE
• Joakim Lundeberg · Lidingö, SE

Key dates

• Filing date: Apr 13, 2012
• Grant date: Jul 24, 2018
• Expiry date: Jan 19, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y600/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to methods and products for the localized or spatial detection of nucleic acid in a tissue sample and in particular to a method for localized detection of nucleic acid in a tissue sample comprising: (a) providing an array comprising a substrate on which multiple species of capture probes are directly or indirectly immobilized such that each species occupies a distinct position on the array and is oriented to have a free 3′ end to enable said probe to function as a primer for a primer extension or ligation reaction, wherein each species of said capture probe comprises a nucleic acid molecule with 5′ to 3′: (i) a positional domain that corresponds to the position of the capture probe on the array, and (ii) a capture domain; (b) contacting said array with a tissue sample such that the position of a capture probe on the array may be correlated with a position in the tissue sample and allowing nucleic acid of the tissue sample to hybridize to the capture domain in said capture probes; (c) generating DNA molecules from the captured nucleic acid molecules using said capture probes as extension or ligation primers, wherein said extended or ligated DNA molecule…