Modified transposases for improved insertion sequence bias and increased DNA input tolerance

US10035992B2 · kind B2 · utility

Assignee

• Illumina, Inc. · US

Inventors

• Christian Gloeckner · Bonn, DE
• Amirali Kia · San Mateo, US
• Erin Bomati · Santee, US
• Molly He · San Diego, US
• Haiying Li Grunenwald · Belleville, US
• Scott Kuersten · Madison, US
• Trina Faye Osothprarop · San Diego, US
• Darin Haskins · Madison, US
• Joshua Burgess · Sun Prairie, US
• Anupama Khanna · Madison, US
• Daniel Schlingman · Madison, US
• Ramesh Vaidyanathan · Madison, US

Key dates

• Filing date: Oct 12, 2017
• Grant date: Jul 31, 2018
• Expiry date: Oct 12, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.