Primer middle sequence interference PCR technology

US10036062B2 · kind B2 · utility

Assignee

• BEIJING TAG-ARRAY MOLECULAR TEST CO., LTD. · CN

Inventors

• Hong Jiang · Nanhu, CN
• Suwen Yue · Beijing, CN
• Tongbing Liao · Beijing, CN
• Bisheng Jiang · Beijing, CN
• Yue Qu · Zhuhai, CN
• Yukang Jiang · Beijing, CN

Key dates

• Filing date: Nov 28, 2013
• Grant date: Jul 31, 2018
• Expiry date: Nov 28, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6848
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a primer middle sequence interference PCR method, and the method uses one segment of a non-complemented or same-sequence base of the middle sequence of primers to perform antisense interference inside and outside the primer molecules, so as to competitively destroy the polymerization among the primers to selectively inhibit amplification of the primer dimer (PD).