Method for determining an analyte content of a liquid sample by means of a bioanalyzer

US10036098B2 · kind B2 · utility

Assignee

• Endress+Hauser Conducta GmbH+Co. KG · DE

Inventors

• Michael Hanko · Dresden, DE
• Angela Eubisch · Chemnitz, DE
• Axel Fikus · Hartha, DE

Key dates

• Filing date: Aug 26, 2011
• Grant date: Jul 31, 2018
• Expiry date: Aug 27, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/56983
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for automated in situ determining an analyte content of a liquid sample by means of a bioanalyzer, wherein a measurement duct has at least one substrate, comprising a repeatedly performable sequence of steps as follows: (i) preparing a sensor matrix, which has a plurality of receptors, which bind the analyte and/or a further target molecule, or bring about a chemical conversion of the analyte or of the further target molecule, leading through the measurement duct a preparation solution of at least a first chemical species, wherein a plurality of the first chemical species are bound on the substrate via the functional group binding on the substrate, wherein the other functional group of the plurality of the first chemical species bound on the substrate serves as a receptor or for subsequent binding of a receptor; (ii) leading the liquid sample, through the measurement duct, wherein analyte contained in the liquid sample or in the liquid to be measured, and/or other target molecules contained in the liquid sample or the liquid to be measured, bind, preferably selectively and specifically, on the receptors or are chemically converted by the receptors, and determining a measur…