Solution phase homogeneous assays

US10036708B2 · kind B2 · utility

Assignee

• Beckman Coulter, Inc. · US

Inventors

• Hashem Akhavan-Tafti · Howell, US
• Dean G. Binger · St. Louis Park, US
• Ying Chen · Eden Prairie, US
• Renuka de Silva · Northville, US
• Terri McLernon · Mounds View, US
• James Mendoza · Robbinsdale, US
• Bruce H. Odegaard · Crystal, US
• Michael Salvati · Minnetrista, US
• Nir Shapir · Falcon Heights, US
• Wenhua Xie · Miaocheng, CN

Key dates

• Filing date: Apr 2, 2015
• Grant date: Jul 31, 2018
• Expiry date: Feb 24, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/582
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.