Codon optimized recombinant phage and methods of using same

US10039795B2 · kind B2 · utility

Assignee

• Institute for Environmental Health, Inc. · US

Inventors

• Michael Sandor Koeris · Natick, US
• Jayson Bowers · Cambridge, US
• Daniel Robert Brownell · Arlington, US
• Edyta Krzymanska-Olejnik · Brookline, US
• Robert Patrick Shivers · Watertown, US
• Michael Cappillino · Reading, US

Key dates

• Filing date: May 8, 2015
• Grant date: Aug 7, 2018
• Expiry date: Jul 18, 2035

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is Listeria.