Culture method of embryoid bodies and/or neural stem cells derived from human differentiated cell-derived pluripotent stem cells
US10041040B2 · kind B2 · utility
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Key dates
| Filing date | Feb 3, 2010 |
| Grant date | Aug 7, 2018 |
| Priority date | — |
| Expiry date | Feb 3, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/45
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method for differentiating a human differentiated cell-derived pluripotent stem cell into a neural stem cell is provided, which includes the steps of: making an embryoid body from the human differentiated cell-derived pluripotent stem cell; and culturing the embryoid body in a medium containing LIF to differentiate into a neural stem cell, so that, when the neural stem cell is allowed to differentiate in vitro after multiple subculturing of the neural stem cell, it differentiate mainly into neurons but substantially not into glial cells.
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