Rationally-designed single-chain meganucleases with non-palindromic recognition sequences
US10041053B2 · kind B2 · utility
11Cited by
17References
11Claims
0Family size
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Key dates
| Filing date | Apr 19, 2016 |
| Grant date | Aug 7, 2018 |
| Priority date | — |
| Expiry date | Aug 11, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are rationally-designed, non-naturally-occurring meganucleases in which a pair of enzyme subunits having specificity for different recognition sequence half-sites are joined into a single polypeptide to form a functional heterodimer with a non-palindromic recognition sequence. The invention also relates to methods of producing such meganucleases, and methods of producing recombinant nucleic acids and organisms using such meganucleases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.