Methods and apparatus for synthesizing nucleic acids
US10041110B2 · kind B2 · utility
4Cited by
18References
23Claims
0Family size
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Key dates
| Filing date | Jul 28, 2014 |
| Grant date | Aug 7, 2018 |
| Priority date | — |
| Expiry date | Feb 28, 2036 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/582
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using enzymes and specially designed nucleotide analogs. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template. Because the nucleotide analogs have an unmodified 3′ OH, i.e., as found in “natural” deoxyribose and ribose molecules, the analogs result in natural polynucleotides suitable for incorporation into biological systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.