Methods of purifying antibodies
US10047144B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 19, 2012 |
| Grant date | Aug 14, 2018 |
| Priority date | — |
| Expiry date | Jan 18, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/52
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods of purifying antibodies using various antibody-specific purification media to rapidly and efficiently separate mixtures of antibodies, antibody fragments and/or antibody components to isolate a desired antibody product from the mixture. The invention relates to the purification of bispecific monoclonal antibodies carrying a different specificity for each binding site of the immunoglobulin molecule, e.g., antibodies composed of a single heavy chain and two different light chains, one containing a Kappa constant domain and the other a Lambda constant domain, including antibodies of different specificities that share a common heavy chain. The invention also provides the methods of efficiently purifying intact antibodies by separating the intact antibody from non-intact antibodies including free light chains.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.