Citrus derived transcription and translation regulatory elements for tissue specific expression

US10047369B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary of Agriculture · US

Inventors

• William R. Belknap · Albany, US
• Kent F. McCue · Corona, US
• Roger L. Thilmony · Corona, US
• Eddie W Stover · Fort Pierce, US
• James G. Thomson · Corona, US

Key dates

• Filing date: Dec 16, 2016
• Grant date: Aug 14, 2018
• Expiry date: Feb 28, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8235
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Expression vectors and expression cassettes containing a tissue specific 5′ transcription regulatory element, optionally linked to a translation regulatory element, optionally linked to an intron transcription regulatory element, operably linked to a heterologous polynucleotide encoding a protein or RNA of interest are described. The 5′ transcription regulatory elements control expression in root tissue cells, phloem tissue cells, or fruit and/or abscission zone tissue cells. These sequences are obtained from citrus plants. Methods of use of these expression vectors and expression cassettes are described, as well as genetically altered plants and parts thereof that contain these expression vectors and/or cassettes.