Patent · US Active

Channels with cross-sectional thermal gradients

US10052632B2 · kind B2 · utility

0Cited by
80References
17Claims
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Assignee

Inventors

Key dates

Filing dateDec 28, 2015
Grant dateAug 21, 2018
Priority date—
Expiry dateFeb 28, 2036

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01L2400/086
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid annealing zone, and a nucleic acid polymerization zone. Such devices find use in, for example, nucleic acid amplification procedures, including digital polymerase chain reaction (dPCR) to temperature cycle droplets for amplification of nucleic acid templates within the droplets.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.