Enrichment and isolation of microbial cells and microbial nucleic acids from a biological sample
US10053722B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 20, 2012 |
| Grant date | Aug 21, 2018 |
| Priority date | — |
| Expiry date | Sep 6, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for enrichment and isolation of microbial cells and microbial nucleic acids from a biological sample is described. The method comprises (i) adding to an initial volume of biological sample a differential cell lysis solution to obtain a final concentration of 0.1 to 1% of SDS in the sample; (ii) mixing the solution obtained in step (i) for a period of time sufficient to lyse the host cells present in the biological sample, while preserving the integrity of cells; and (iii) separating the microbial cells from the lysed host cells components. Differential cell lysis solutions and kits for practicing the method of the present invention are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.