Modified template-independent enzymes for polydeoxynucleotide synthesis
US10059929B2 · kind B2 · utility
23Cited by
1References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 20, 2015 |
| Grant date | Aug 28, 2018 |
| Priority date | — |
| Expiry date | Mar 16, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07031
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention includes methods for identifying polymerases, such as modified terminal nucleotidyl transferases (TdT), that are capable of binding nucleotides comprising removable 3′-O-blocking moieties to a nucleic acid initiator, without the use of a template. The invention further includes the identified polymerases, and methods of using the polymerases for de novo synthesis of predetermined oligonucleotide sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.