In situ nucleic acid sequencing of expanded biological samples

US10059990B2 · kind B2 · utility

Assignee

• Massachusetts Institute of Technology · US

Inventors

• Edward S. Boyden · Sharon, US
• Fei Chen · Thạnh Trung, VN
• Shahar Alon · Cambridge, US
• George M. Church · Brookline, US
• Paul Warren Tillberg · Cambridge, US
• Adam Marblestone · Arlington, US
• Evan R. Daugharthy · Cambridge, US

Key dates

• Filing date: Apr 14, 2016
• Grant date: Aug 28, 2018
• Expiry date: Jun 20, 2036

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2300/043
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides in situ nucleic acid sequencing to be conducted in biological specimens that have been physically expanded. The invention leverages the techniques for expansion microscopy (ExM) to provide new methods for in situ sequencing of nucleic acids as well as new methods for fluorescent in situ sequencing (FISSEQ) in a new process referred to herein as “expansion sequencing” (ExSEQ).