Use of anti-CD47 agents to enhance immunization

US10064925B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Diane Tseng · San Jose, US
• Jens-Peter Volkmer · Menlo Park, US
• Kipp Andrew Weiskopf · Sudbury, US
• Stephen Willingham · Stanford, US
• Irving L. Weissman · Palo Alto, US

Key dates

• Filing date: Apr 23, 2014
• Grant date: Sep 4, 2018
• Expiry date: May 16, 2034

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2501/998
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods are provided for enhancing immunization strategies by manipulation, e.g. in vitro manipulation, of phagocytic antigen presenting cells. In the methods of the invention, phagocytic antigen presenting cells (phAPC) are incubated with a particulate antigen in the presence of an anti-CD47 agent in a dose and for a period of time sufficient to allow the phAPC to phagocytose the particulate antigen, which process generates a “loaded” phAPC. The loaded phAPC is contacted with a population of T cells matched for at least one major histocompatibility locus with the phAPC, where the T cells are stimulated after contacting to generate an effector response against an epitope or epitopes present on the particulate antigen.