Method of monitoring the release from liposomes of a product of interest using superparamagnetic nanoparticles
US10064962B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 19, 2015 |
| Grant date | Sep 4, 2018 |
| Priority date | — |
| Expiry date | Oct 19, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01R33/5601
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention pertains to a method of monitoring the membrane permeabilization of a liposome and the incidental release of a compound of interest. The method utilizes liposomes comprising a thermosensitive lipidic membrane encapsulating the product of interest and superparamagnetic nanoparticles having the electrostatic surface charge below −20 mV or above +20 mV when measured in an aqueous medium at physiological pH. In one embodiment, the method comprises the steps of: a) measuring relaxation time (T2*); b) heating the liposome at Tm or above Tm; c) measuring T2* after step b); d) obtaining the transverse relaxivity (r2*) values from the T2* values obtained from step a) and step c); and e) determining the ratio of r2* before and after the heating step b). A ratio above 1.5 indicates the liposome membrane permeabilization and the incidental release of the product of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.