Nanofluidic sorting system for gene synthesis and pcr reaction products
US10065154B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 4, 2013 |
| Grant date | Sep 4, 2018 |
| Priority date | — |
| Expiry date | Oct 9, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2015/1028
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Devices and methods integrate nanopore and microfluidic technologies for recording molecular characteristics of individual molecules such as, for example, biomolecules. Devices comprise a first substrate comprising a microchannel, a second substrate comprising a microchannel, the second substrate positioned below the first substrate, and a membrane having a thickness of about 0.3 nm to about 1 nm and comprising at least one nanopore, the membrane positioned between the first substrate and the second substrate, wherein a single nanopore of the membrane is constructed and arranged for electrical and fluid communication between the microchannel of the first substrate and the microchannel of the second substrate. To mitigate the effect of errors that occur during de novo DNA synthesis, longer DNA molecules are typically synthesized from shorter oligonucleotides by polymerase construction and amplification (PCA), or by other methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.