Patent · US Active

High pH protein refolding methods

US10065987B2 · kind B2 · utility

2Cited by

17References

16Claims

0Family size

Assignee

Bristol-Myers Squibb Company · US

Inventors

Cristopher Hollander · Newton, US
Benjamin C. Blum · Boston, US

Key dates

Filing date	Feb 11, 2014
Grant date	Sep 4, 2018
Priority date	—
Expiry date	Oct 5, 2034

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/70
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided herein are methods for refolding denatured protein (e.g., from inclusion bodies) that do not require the use of a denaturing agent. Exemplary methods use a high pH for solubilizing denatured protein, followed by a decrease in pH for refolding the proteins.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.