Methods, surface modified plates and compositions for cell attachment, cultivation and detachment
US10066203B2 · kind B2 · utility
3Cited by
78References
22Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Feb 19, 2009 |
| Grant date | Sep 4, 2018 |
| Priority date | — |
| Expiry date | Sep 12, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.