Method for purifying and quantifying thrombin and its degradation polypeptides
US10066224B2 · kind B2 · utility
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12Claims
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Key dates
| Filing date | Feb 24, 2016 |
| Grant date | Sep 4, 2018 |
| Priority date | — |
| Expiry date | Feb 24, 2036 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/96463
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Provided is a method for purifying α-thrombin and for quantifying α-thrombin and its degradation polypeptides in a liquid proteinatious solution. The method employs a one-step anion exchange chromatography method. The method allows purification and/or quantification of a homogenous post-translationally modified α-thrombin. The method can also be used for purification and/or quantification of β-thrombin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.