E. coli separatome-based protein expression and purification platform

US10066232B2 · kind B2 · utility

Assignees

• Board of Trustees of the University of Arkansas · US
• University of Pittsburgh—Of the Commonwealth System of Higher Education · US

Inventors

• Ellen M. Brune · Fayetteville, US
• Robert Beitle · Fayetteville, US
• Mohammad M. Ataai · Pittsburgh, US
• Patrick R. Bartlow · Pittsburgh, US
• Ralph Henry · Fayetteville, US

Key dates

• Filing date: Oct 24, 2017
• Grant date: Sep 4, 2018
• Expiry date: Oct 24, 2037

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a separatome-based peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome of E. coli. The separatome-based protein expression and purification platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the separatome-based protein expression and purification platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies facilitating efficient product purification. The separatome-based protein expression and purification platform is an efficient bioseparation system that intertwines host cell expression systems and chromatography.